Invitrogen™ DKK1 Mouse ELISA Kit

Beschreibung

The Mouse Dkk-1 ELISA quantitates Ms Dkk-1 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms Dkk-1. Principle of the method The Mouse Dkk-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Dieses Gen kodiert ein Protein, das zur Dickkopf-Familie gehört. Es handelt sich um ein sezerniertes Protein mit zwei Zystein-reichen Regionen und ist durch seine Hemmung des WNT-Signalwegs an der embryonalen Entwicklung beteiligt. Erhöhte DKK1-Konzentrationen im Knochenmarkplasma und im peripheren Blut sind mit dem Vorhandensein osteolytischer Knochenläsionen bei Patienten mit multiplem Myelom verbunden.