Invitrogen™ Endocan (ESM1) Mouse ELISA Kit

Beschreibung

The Mouse Endocan (Ms ESM1) ELISA quantitates Ms Endocan in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms Endocan. Principle of the method The Mouse Endocan solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Dieses Gen codiert ein sezerniertes Protein, das hauptsächlich in den endothelialen Zellen im menschlichen Lungen- und Nierengewebe exprimiert wird. Die Expression dieses Gens wird durch Zytokine reguliert, was darauf hindeutet, dass es bei Endothelium-abhängigen pathologischen Erkrankungen eine Rolle spielt. Das Transkript enthält mehrere Polyadenylierungs- und mRNA-Instabilitätssignale. Für dieses Gen wurden zwei Transkriptvarianten gefunden, die unterschiedliche Isoformen codieren.