Invitrogen™ MFG-E8 Mouse ELISA Kit

Beschreibung

The Mouse MFG-E8 ELISA quantitates Ms MFG-E8 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms MFG-E8. Principle of the method The Mouse MFG-E8 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Humanes Milchfettglobul-Membranprotein (HMFG1) ist auf normalen humanen Brustepithelzellen und Zelllinien vorhanden, die von Brustkarzinomen abgeleitet werden, sowie auf der Außenfläche der humanen Milch-Fettkugel. HMFG1 gilt als Differenzierungsmarker. Es ist als spezifischer Brustepithelmarker bei der normalen und neoplastischen Entwicklung der Milchsäugetiere nützlich.