Invitrogen™ PLGF-2 (PGF) Mouse ELISA Kit

Beschreibung

The Mouse PLGF-2 (PGF) ELISA quantitates Ms PLGF-2 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms PLGF-2. Principle of the method The Mouse PLGF-2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Der plazentale Wachstumsfaktor (PlGF) ist ein Mitglied der VEGF-Familie (Vaskulärer endothelialer Wachstumsfaktor). PlGF ist ein pleiotroper Faktor, der verschiedene Zelltypen beeinflusst und diverse biologische Reaktionen reguliert. Die Aktivität des vaskulären endothelialen Wachstumsfaktors (VEGF), der der stärkste aller angiogenen Mediatoren ist, wird zum Teil von PlGF moduliert. Dies spielt eine wichtige Rolle bei der Vaskulogenese und Angiogenese in der menschlichen Plazenta.