Invitrogen™ Novex™ phosphoELISA™ 4E-BP1 [pT46] ELISA Kit

Beschreibung

The 4E-BP1 [pT46] ELISA kit is intended for the detection of 4E-BP1 [pT46] from mouse, human and rat cell extracts using 96-well plates and a microplate reader.

Easy-to-Run Sandwich ELISA
A monoclonal capture antibody specific for 4E-BP1 has been coated onto the wells of the 96-well plate provided. During the first incubation, standards of known 4E-BP1 [pT46] content and unknown samples are pipetted into the wells and the 4E-BP1 antigen binds to the immobilized (capture) antibody. After washing, a rabbit antibody specific for 4E-BP1 phosphorylated at threonine 46 is added to the wells. During the second incubation, this antibody serves as a detection antibody by binding to the immobilized 4E-BP1 protein captured during the first incubation. After washing, a horseradish peroxidase labeled anti-rabbit IgG is added. This binds to the detection antibody to complete the four member sandwich. After a third incubation and washing to remove all the unbound enzyme, a substrate solution (TMB) is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of 4E-BP1 [pT46] present in the original specimen and the optical density can be read on a standard microplate reader. The total assay incubation time is only four hours.

Detect Low Expressing Proteins and Use Less Sample
ELISA kits designed to measure intracellular signaling targets are 2–10X more sensitive than Western blotting. The improved sensitivity enables you to detect low-expressing proteins that otherwise may not be distinguishable from background. In addition, the amount of sample needed to run the assay is less than what is needed for Western blots.

Convenient Kit Format
The kit comes with a capture antibody precoated to the plate, eliminating an overnight coating process. The plates are machine coated to provide low variability well-to-well. Furthermore, the plate comes as removable 8-well strips allowing you to run as few samples as you want. In addition, the standards provided in the kit can be used to obtain quantitative results from the standard curve and/or can be used as a positive control.

Rigorous Validation
Each ELISA kit is validated for sensitivity, specificity, precision, and lot-to-lot consistency. See protocol insert for more information on validation.

• Easy to run
• More sensitive than Westerns
• Convenient precoated, removable 8-well strips

Cell Analysis, Drug Discovery and Development, Immunoassays, Kinase Antibodies and Immunoassays, Kinase Biology, Ready-To-Use Immunoassay, Target and Lead Identification and Validation

Bestellinformation

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