Invitrogen™ Phospho-CD247 (CD3 zeta) (Tyr142) Monoclonal Antibody (3ZBR4S), APC, eBioscience™

Mouse Monoclonal Antibody

This 3ZBR4S monoclonal antibody recognizes human and mouse CD247 (CD3z) when phosphorylated on Tyrosine 142. CD3 is a multi-protein complex expressed on the surface of thymocytes and T cells, a marker of the T cell lineage, and a critical component of T cell receptor (TCR) signaling. The complex contains a gamma chain, a delta chain, and two epsilon chains, which associate with the alpha/beta or gamma/delta chains of the TCR, and the zeta chain homodimer. The CD3 zeta chain is responsible for initiating the signaling cascade following engagement of the TCR. Each CD3 zeta chain contains three immunoreceptor tyrosine-based activation motifs (ITAM) that are phosphorylated by Src kinases such as Lck. Once phosphorylated, these ITAMs form docking sites for SH2 domain-containing proteins and function to recruit ZAP-70 to the TCR where it can further amplify and propagate signaling. Loss or mutation of CD3 zeta or the ITAM motifs results in impaired T cell development, signaling, and function. CD3 zeta phosphorylation occurs early in the TCR signaling cascade. It occurs immediately downstream of Lck activation, and upstream of ZAP-70, LAT, and SLP-76. Applications Reported: This 3ZBR4S antibody has been reported for use in flow cytometric analysis. Applications Tested: This 3ZBR4S antibody has been pre-diluted and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This may be used at 5 μL (0.25 μg) per test. A test is defined as the...