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Invitrogen™ Phospho-gamma-H2AX (Ser139) Recombinant Rabbit Monoclonal Antibody (BLR053F)

Beschreibung
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
H2AX is a variant of the H2A protein family and is a component of the histone octomer in nucleosomes. H2AX is phosphorylated by kinases such as ataxia telangiectasia mutated (ATM) and ATM-Rad3-related (ATR) in the PI3K pathway. Gamma-H2AX is the first step in recruiting and localizing DNA repair proteins. DSBs can be induced by mechanisms such as ionizing radiation or cytotoxic agents and subsequently, gamma-H2AX foci quickly form. These foci represent the DSBs in a 1:1 manner and can be used as a biomarker for damage. The visualization of gamma-H2AX by can allow the assessment of DNA damage, related DNA damage proteins and DNA repair. Gamma-H2AX has applications in the detection of genomic damage caused by cytotoxic chemical agents and environmental and physical damage.
Spezifikation
Spezifikation
| Antigen | Phospho-gamma-H2AX (Ser139) |
| Anwendungen | Flow Cytometry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry |
| Klassifikation | Recombinant Monoclonal |
| Klon | BLR053F |
| Konzentration | 100 μg/mL |
| Konjugat | Unconjugated |
| Zusammensetzung | BBS with 0.1% BSA and 0.09% sodium azide; pH 8.2 |
| Gen | H2AX |
| Gen-Zugriffsnummer | P16104 |
| Gen-Alias | gamma H2A X; gamma H2A.X; gamma-H2A.X; gh2ax |
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