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R&D Systems™ Recombinant Human LIGHT/TNFSF14 Protein
Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity
N.V.
Spezifikation
| Zugriffsnummer | O43557 |
|---|---|
| Zur Verwendung mit (Anwendung) | Bioactivity |
| Gen-ID (Entrez) | 8740 |
| Molekulargewicht | M.W. (Observed): 25 kDa, reducing conditions; M.W. (theoretical): 20.9 kDa |
| Name | LIGHT/TNFSF14 |
Beschreibung
Measured in a cytotoxicity assay using HT-29 human colon adenocarcinoma cells Yu, K.Y. et al. (1999) J. Biol. Chem. 274:13733; Harrop, J.A. et al. (1998) J. Biol. Chem. 273:27548; Zhai, Y. et al. (1998) J. Clin. Invest. 102:1142. Recombinant Human LIGHT/TNFSF14 at 10 ng/mL causes a 2-4.5 fold inhibition of HT29 proliferation, in the presence of 10 U/mL (1 ng/mL) Recombinant Human IFN-gamma (Catalog # 285-IF ). Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323. The ED50 for this effect is 1-4 ng/mL.Spezifikation
| O43557 | |
| 8740 | |
| LIGHT/TNFSF14 | |
| Mouse myeloma cell line, NS0-derived human LIGHT/TNFSF14 protein Met 10-His tag GGGSGGGSGGGSIEGR Human Light (Asp74-Val240) (N-terminus) Accession # O43557 (C-terminus) | |
| <0.10 EU / 1 μg of the protein by the LAL method. | |
| Unconjugated | |
| >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining. |
| Bioactivity | |
| M.W. (Observed): 25 kDa, reducing conditions; M.W. (theoretical): 20.9 kDa | |
| 25 μg | |
| Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution. | |
| TNFSF14 | |
| Recombinant | |
| LIGHT/TNFSF14 |