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R&D Systems™ Human LIGHT/TNFSF14 Recombinant Protein

Marke:  R&D Systems™ 664-LI-025/CF

Weitere Details : Netto-Gewicht : 0.00010kg

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Artikelnummer. 13415894

  • 487.52 € / 25 Mikrogramm

Voraussichtlicher Liefertermin 02-05-2019
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Measured in a cytotoxicity assay using HT-29 human colon adenocarcinoma cells Yu, K.Y. et al. (1999) J. Biol. Chem. 274:13733; Harrop, J.A. et al. (1998) J. Biol. Chem. 273:27548; Zhai, Y. et al. (1998) J. Clin. Invest. 102:1142. Recombinant Human LIGHT/TNFSF14 at 10 ng/mL causes a 2-4.5 fold inhibition of HT29 proliferation, in the presence of 10 U/mL (1 ng/mL) Recombinant Human IFN-gamma (Catalog # 285-IF ). Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323. The ED 50 for this effect is 1-4 ng/mL.


Lyophilized from a 0.2μm filtered solution in PBS with Trehalose.
20.9 kDa
95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Mouse myeloma cell line, NS0-derived Met 10-His tag GGGSGGGSGGGSIEGR Human Light (Asp74-Val240) Accession # O43557
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.