Invitrogen™ Rabbit anti-Chicken IgY (H+L) Secondary Antibody, FITC

Rabbit Polyclonal Secondary Antibody

Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration. This antibody has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications. Antibody Specificity: This antibody reacts with the heavy chains on chicken IgY and with the light chains common to most chicken immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins using immunoelectrophoresis. However, these antibodies may cross-react with immunoglobulins from other species. Restoration and Storage: Store product at 2-8°C until opened. Restore to 1.1 mL with distilled water (1.5 mg/mL after restoration). Centrifuge product if it is not clear upon standing for 1-2 hours at room temperature. To judge clarity, draw the product into a pasteur pipette. Product may be stored for several weeks at 2-8°C as undiluted liquid. After dilution, do not use for more than one day. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. Fluorophore: Fluorescein-5-isothiocyanate (FITC-isomer 1) Amax = 492 nm; Emax = 520 nm Fluorophore/Protein: 8.5 μg/mg; 3.2 moles FITC per mole IgG Country of Origin: USA.