Invitrogen™ Lipocalin-2 (LCN2/NGAL) Rat ELISA Kit

Beschreibung

The Rat Lipocalin-2 (LCN2/NGAL) ELISA quantitates Rt Lipocalin-2 in rat serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Rt Lipocalin-2. Principle of the method The Rat Lipocalin-2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

NGAL (Neutrophiles Gelatinase-assoziiertes Lipocalin, Lipocalin2, Siderokalin und neutrophiles Lipocalin) ist ein einkettiges glykosyliertes Monomer mit 25 kDa und Mitglied der Lipocalin-Protein-Familie, die kleine lipophile Moleküle binden und transportieren. NGAL wird durch aktivierte Neutrophile freigesetzt, kann Dimere und kleine Mengen höherer Oligomere bilden und Komplexe mit Matrixmetalloproteinase 9 (MMP-9, Gelatinase B) eingehen. Eine geringe Expression von NGAL in einer Vielzahl von Epithelien kann bei Entzündungen oder Krebserkrankungen erhöht sein. Die Expression von NGAL in der Niere wird durch ischämische oder nephrotoxische Nierenschädigungen drastisch erhöht.