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enQuireBio™ Recombinant Chicken GH Antagonist Protein
A cDNA sequence encoding the GH Antagonist was constructed and used to recombinantly synthesize the protein.
Marke: enQuireBio™ QP10641-100ug
Weitere Details : Netto-Gewicht : 0.01000kg
Spezifikation
| GH Antagonist Protein | |
| Research Use Only | |
| Recombinant Chicken Growth Hormone G119R mutant did not bind to ovine GHR-ECD and was devoid of any biological activity in FDC-P1 3B9 cells. However, in binding experiments that were carried out using chicken liver membranes, both ovine GH and chicken GH showed similar IC50 values in competition with 125I-ovine GH, while the IC50 of G119R mutein was 10-fold higher. These results emphasize the importance of species specificity and indicate the possibility of antagonistic activity of chGH G119R in homologous system. | |
| Chicken | |
| Untagged | |
| Greater than 98.0% as determined by:(a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE. |
| 100 μg | |
| < 1.0 EU per ug protein as determined by the LAL method. | |
| Recombinant Protein | |
| E. coli | |
| The protein was lyophilized from a concentrated (1 mg/ml) solution with 0.3% NaHCO3 pH 8. |