missing translation for 'onlineSavingsMsg'
Learn More
Learn More
enQuireBio™ Recombinant Gilthead Seabream IGF1 Protein
A cDNA sequence encoding the IGF1 was constructed and used to recombinantly synthesize the protein.
137.00 € - 3180.00 €
Spezifikation
| Name | IGF1 Protein |
|---|---|
| Kennzeichnung | Research Use Only |
| Endotoxin-Konzentration | < 1.0 EU per ug protein as determined by the LAL method. |
| Biologische Aktivität | Binding assays of the 125I-Gealthead Seabream IGF1 to Gilthead Seabream or carp (Cyprinus carpio) sera resulted in high specific binding, indicating the existence of one or more IGF-binding proteins. In binding experiments to crude Gilthead Seabream brain homogenate, using human (h) IGF-I as a ligand, the respective IC50 value of hIGF1 was about fourfold lower than that of Gilthead Seabream IGF-1. Recombinant Gilthead Seabream IGF-1 exhibited mitogenic activity in a mouse mammary gland-derived MME-L1 cell line which was approximately 200-fold lower than that of hIGF1. Binding experiments to intact MME-L1 cells suggests that this difference most likely results from a correspondingly lower affinity for IGF1 receptor in these cells. In contrast, the activities of Gilthead Seabream IGF-I and hIGF-I measured by 35S uptake by gill arches from the goldfish (Carassius auratus) were identical, indicating that the recombinant Gilthead Seabream IGF-I is biologically active. |
| Produkttyp | Recombinant Protein |
| Produktcode | Marke | Menge | Preis | Menge & Verfügbarkeit | |||||
|---|---|---|---|---|---|---|---|---|---|
| Produktcode | Marke | Menge | Preis | Menge & Verfügbarkeit | |||||
|
15914628
|
enQuireBio™
QP10678-10UG |
10 μg |
137.00 €
10 Mikrogramm |
Verfügbar ab: 24-06-2024 Loggen Sie sich ein, um den Lagerbestand zu sehen |
Bitte melden Sie sich an, um diesen Artikel zu kaufen. Sie benötigen ein Web-Konto? Registrieren Sie sich noch heute! | ||||
|
15934628
|
enQuireBio™
QP10678-50UG |
50 μg |
212.00 €
50 Mikrogramm |
Verfügbar ab: 24-06-2024 Loggen Sie sich ein, um den Lagerbestand zu sehen |
Bitte melden Sie sich an, um diesen Artikel zu kaufen. Sie benötigen ein Web-Konto? Registrieren Sie sich noch heute! | ||||
|
15924628
|
enQuireBio™
QP10678-1MG |
1 mg |
3180.00 €
1 Milligramm |
Verfügbar ab: 24-06-2024 Loggen Sie sich ein, um den Lagerbestand zu sehen |
Bitte melden Sie sich an, um diesen Artikel zu kaufen. Sie benötigen ein Web-Konto? Registrieren Sie sich noch heute! | ||||
Spezifikation
| IGF1 Protein | |
| < 1.0 EU per ug protein as determined by the LAL method. | |
| Recombinant Protein | |
| E. coli | |
| The protein was lyophilized from a concentrated (1 mg/ml) solution with 0.02% NaHCO3. |
| Research Use Only | |
| Binding assays of the 125I-Gealthead Seabream IGF1 to Gilthead Seabream or carp (Cyprinus carpio) sera resulted in high specific binding, indicating the existence of one or more IGF-binding proteins. In binding experiments to crude Gilthead Seabream brain homogenate, using human (h) IGF-I as a ligand, the respective IC50 value of hIGF1 was about fourfold lower than that of Gilthead Seabream IGF-1. Recombinant Gilthead Seabream IGF-1 exhibited mitogenic activity in a mouse mammary gland-derived MME-L1 cell line which was approximately 200-fold lower than that of hIGF1. Binding experiments to intact MME-L1 cells suggests that this difference most likely results from a correspondingly lower affinity for IGF1 receptor in these cells. In contrast, the activities of Gilthead Seabream IGF-I and hIGF-I measured by 35S uptake by gill arches from the goldfish (Carassius auratus) were identical, indicating that the recombinant Gilthead Seabream IGF-I is biologically active. | |
| Gilthead Seabream | |
| Untagged | |
| Greater than 98.0% as determined by:(a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE. |