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enQuireBio™ Recombinant Mouse DHFR Protein
A cDNA sequence encoding the DHFR was constructed and used to recombinantly synthesize the protein.
Marke: enQuireBio™ QP10575-20ug
Weitere Details : Netto-Gewicht : 0.01000kg
Spezifikation
| DHFR Protein | |
| Research Use Only | |
| Specific activity is > 0.2 units/mg, in which one unit will convert 1.0 umole of 7,8 –dihydrofloate and beta-NADPH to 5,6,7,8-tetrahydrofloate and beta-NADP per min at pH 6.5 at 25C. | |
| Mouse | |
| His | |
| The DHFR solution (1 mg/ml) contains 20mM Tris-HCl buffer(pH 8.0), 10% glycerol, 2mM DTT and 0.1M NaCl. |
| 20 μg | |
| < 1.0 EU per ug protein as determined by the LAL method. | |
| Recombinant Protein | |
| E. coli | |
| MGSSHHHHHH SSGLVPRGSH MVRPLNCIVA VSQNMGIGKN GDLPWPPLRN EFKYFQRMTT TSSVEGKQNL VIMGRKTWFS IPEKNRPLKD RINIVLSREL KEPPRGAHFL AKSLDDALRL IEQPELASKV DMVWIVGGSS VYQEAMNQPG HLRLFVTRIM QEFESDTFFP EIDLGKYKLL PEYPGVLSEV QEEKGIKYKF EVYEKKD | |
| Greater than 95.0% as determined by SDS-PAGE. |