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R&D Systems™ Recombinant TEV Protease Protein
Description
Measured by its ability to cleave a fusion protein containing the recognition sequence Glu-Asn-Leu-Tyr-Phe-Gln, with the cleavage point after Gln. TEV Protease cleaves =50% of the control substrate, as measured under the described conditions. It is recommended that the cleavage for each fusion protein be optimized by varying the amount of Recombinant Viral TEV Protease, reaction time, or incubation temperature.
Specifications
Specifications
| Zugriffsnummer | NP_062908 |
| Zur Verwendung mit (Anwendung) | Enzyme Activity |
| Zusammensetzung | Supplied as a 0.2 μm filtered solution in Tris, NaCl, EDTA, DTT and Glycerol. |
| Gen-ID (Entrez) | 1502321 |
| Molekulargewicht | M.W. (Observed): 25-30 kDa, reducing conditions; M.W. (theoretical): 28.5 kDa |
| Name | Sleeping Beauty Transposase |
| Menge | 200 μg |
| Quelle | E. coli-derived viral TEV Protease protein Ala TEV Protease Glu2039-Gln2279 (Ser2256Asn) (N-terminus) Accession # NP_062908 Leu 6-His tag (C-terminus) |
| Lagerungsbedingungen | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening. |
| Endotoxin-Konzentration | <1.0 EU / 1 μg of the protein by the LAL method. |
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