EGF Recombinant Human Protein, Invitrogen™ Sino Biological™

Beschreibung

Epidermal growth factor (EGF) recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.
Background: Human epidermal growth factor (EGF) exerts actions on a wide variety of biological processes, and plays an important role in the regulation of cell growth, proliferation, and differentiation mediated by its high-affinity receptor EGFR on the cell surface. It induces differentiation of specific cells, promotes angiogenesis, and inhibits gastric acid secretion in vivo, while serving as a potent mitogenic actor for a variety of cultured cells of both ectodermal and mesodermal origin. Human EGF was originally isolated from urine based on its inhibitory effect on gastric secretion and named urogastrone accordingly. EGF is the founding member of the EGF-family of proteins which are characterized by the presence of at least one EGF structural unit (a conserved six cysteine motif that forms three disulfide bonds). The EGF precursor is believed to exist as a membrane-bound molecule containing nine EGF units, and is proteolytically cleaved to generate the 53-amino acid peptide hormone.

• N-terminal Sequence Analysis: Met 1
• Activity: Measured in a cell proliferation assay using BALB/c 3T3 mouse embryonic fibroblasts (Rubin, J.S. et al. 1991, Proc. Natl. Acad. Sci. USA 88:415.), the EC₅₀ for this effect is typically 20-100pg/mL
• Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na₂HPO₄, 1.8mM KH₂PO₄, pH 7.4, 5% mannitol, 5% trehalose, 0.02% Tween™-80
• Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible

Manufactured by Sino Biological (China).