missing translation for 'onlineSavingsMsg'
Learn More

Invitrogen™ Human ADAMTS1 Recombinant Protein

Recombinant Protein

Marke:  Invitrogen™ RP77543

Weitere Details : CAS : 69-74-9 Netto-Gewicht : 0.01000kg

Artikelnummer. 11559091

Dieser Artikel ist im Moment nicht verfügbar oder wird nicht mehr hergestellt.
Schauen Sie bitte auf unsere Produktseite nach möglichen Alternativen


Beschreibung

Beschreibung

ADAMTS1 Recombinant Protein, RP-77543, from Invitrogen.

ADAMTS (a disintegrin and metalloproteinase with thrombospondin motif) is a family of secreted proteinases related to, but distinct from ADAM (a disintegrin and metalloproteinase). Presently eighteen family members have been identified in the human genome. ADAMTS1 consists of a prodomain, a metalloprotease domain, a thrombospondin (TSP) homologous domain with TSP type I motif, a spacer region and C-terminal TSP submotifs. The prodomain is cleaved off by a furin-type protease. There occurs additional processing at the C-terminal end. ADAMTS1 has been found in human articular cartilage and in neurons. The protein is induced by IL-1. ADAMTS1 displays antiangiogenic properties and inhibits endothelial cell proliferation. ADAMTS1-null mice exhibit growth retardation and defects in morphology. The active protease cleaves aggrecan at several sites and versican. ADAMTS1 activity is inhibited by metalloproteinase inhibitors TIMP 3 and TIMP 4 at higher concentrations and by catechin gallate esters. RP-77543 is a purified, truncated recombinant human ADAMTS1 protein (amino acids F253-N616 with a C-terminal His tag), expressed in insect cells. Purity is greater than 90% by SDS-PAGE. Calculated Molecular weight is 41 kDa, actual SDS-PAGE results show a band at ∽50 kDa. Specific Activity: Activity of ADAMTS1 is determined with recombinant aggrecan interglobular domain (Product &Num; RP-77527). ADAMTS1 hydrolyzes the "aggrecanase" site within this domain (peptide bond E373 -A374 in human aggrecan). The hydrolysis rate is greater than 0.06 nM hydrolyzed substrate/min x nM truncated ADAMTS1. Permg of enzyme, activity is greater than 1.4 nmoles hydrolyzed substrate/min xmg ADAMTS1. The recombinant substrate is incubated at a concentration of 0.1μM with 0.2-3.0 nM ADAMTS1 in 50 mM Tris-HCL, pH 7.5, 150mM NaCl, 5mM CaCl2, 1 μM leupeptin, 1 μM pepstatin, 1 mM Pefabloc, and 0.05% Brij 35 for 15 min at 37°C. Cleavage is estimated from the appearance of the hydrolysis fragment with N-terminus ARGSVIL. RP-77543 may be used as a control with corresponding anti-ADAMTS1 antibodies. ADAMTS1 is inhibited by chelators of divalent cations such as EDTA and catechin gallate esters.
Spezifikation

Spezifikation

Q9UHI8
TBS with 0.05% Brij-35, 5mM CaCl2 and no preservative; pH 7.5
Human ADAMTS1
5μg
-80°C, Avoid Freeze/Thaw Cycles
Human ADAMTS1
Human
Hu
Recombinant
Recombinant
Control
9510
7.5
Insect Cells
RUO
ADAMTS1
Unconjugated
Baculovirus
Liquid
purified
Produktvorschläge

Produktvorschläge

Videos
Sicherheitsdatenblatt (SDS)
Dokumentation

Dokumentation

Zertifikate
Sonderangebote

Sonderangebote

in vitro research only.