Invitrogen™ Mouse CXCL10 (IP-10) Recombinant Protein

Beschreibung

IP10 (mouse) Recombinant Protein, RP-8668, from Invitrogen.

The chemokine IP-10 (or CXCL10) is a chemokine made by monocytes, endothelial cells and fibroblasts in response to treatment with IFNgamma. IP-10 functions as a chemoattractant to cells expressing the G protein-coupled receptor, CXCR3, which is found mainly on activated T cells and NK cells. IP-10 plays an important role in Th1 type inflammatory diseases and autoimmune diseases such as, Hashimoto's thyroiditis, Graves' disease and Type 1 diabetes mellitus. Recombinant mouse MCP-1 is a non-glycosylated protein, containing 125 amino acids, with a molecular weight of 13.8 kDa. Purity is typically greater than 97% and endotoxin level less than 1 EU/ug. The expected biological activity (ED50) is determined by the ability to chemoattract human PBMCs or THP-1 cells at 4-15 ng/mL. The specific activity of this protein is 2.5x10^5 units/mg. Reconstitute using sterile water at 0.1mg/mL and centrifuge prior to opening vial. Gently pipet solution down the sides of the vial. DO NOT VORTEX sample. Store reconstituted material at -20°C and add 0.1% BSA for additional stability. Amino acid sequence: QPDAVNAPLT CCYSFTSKMI PMSRLESYKR ITSSRCPKEA VVFVTKLKRE VCADPKKEWV QTYIKNLDRN QMRSEPTTLF KTASALRSSA PLNVKLTRKS EANASTTFST TTSSTSVGVT SVTVN.