Invitrogen™ Human TRANCE (RANKL) Recombinant Protein

Beschreibung

soluble RANK-L (human) Recombinant Protein, RP-8630, from Invitrogen.

Receptor Activator of NF-kB Ligand (RANKL) is a cell-bound marker (CD254) related to the TNF family of proteins. RANKL plays a critical role in bone metabolism, particularly osteoclast differentiation. In addition, RANKL is expressed by some T cells is thought to promote dendritic cell maturation. Recombinant soluble human RANKL is a non-glycosylated protein, containing 176 amino acids, with a molecular weight of 20 kDa. Purity is typically greater than 98% and endotoxin level less than 1 EU/ug. The expected biological activity (ED50) is determined by the dose-dependent stimulation of IL-8 production in human PBMC and is typically less than 100 ng/mL. The specific activity of this protein is 1x10^4 units/mg. Reconstitute using sterile water at 0.1mg/mL and centrifuge prior to opening vial. Gently pipet solution down the sides of the vial. DO NOT VORTEX sample. Store reconstituted material at -20°C and add 0.1% BSA for additional stability. Amino acid sequence: MEKAMVDGSW LDLAKRSKLE AQPFAHLTIN ATDIPSGSHK VSLSSWYHDR GWAKISNMTF SNGKLIVNQD GFYYLYANIC FRHHETSGDL ATEYLQLMVY VTKTSIKIPS SHTLMKGGST KYWSGNSEFH FYSINVGGFF KLRSGEEISI EVSNPSLLDP DQDATYFGAF KVRDID.