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  6. Thermo Scientific™ Zeba™ Spin Desalting Columns and Plates, 40K MWCO, 75 μL–10 mL

Thermo Scientific™ Zeba™ Spin Desalting Columns and Plates, 40K MWCO, 75 μL–10 mL

Product Code. 11716446 Shop All Thermo Scientific Products
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Format:
96-well Filter Plate
Spin Column
Volume (Metric):
0.5 mL
10 mL
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75 μL
96-well
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Product Code. Quantity Format Volume (Metric) unitSize
11716446 5 Columns Spin Column 5 mL Pack of 5
11786436 25 Columns Spin Column 75 μL Pack of 25
10754347 50 Columns Spin Column 75 μL Pack of 50
11796436 25 Columns Spin Column 0.5 mL Pack of 25
10460875 50 Columns Spin Column 0.5 mL Pack of 50
11706446 5 Columns Spin Column 2 mL Pack of 5
10329964 25 Columns Spin Column 2 mL Pack of 25
10470875 25 Columns Spin Column 5 mL Pack of 25
11726446 5 Columns Spin Column 10 mL Pack of 5
10753007 25 Columns Spin Column 10 mL Pack of 25
11582521 2 Plates 96-well Filter Plate 96-well Pack of 2
11592521 4 Plates 96-well Filter Plate 96-well Pack of 4
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Product Code. 11716446 Supplier Thermo Scientific™ Supplier No. 87770

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Description

Effectively remove salt and other molecules (40K MWCO) from samples and attain excellent protein recovery with ready-to-use Thermo Scientific Pierce Zeba desalting chromatography columns and plates, which feature a proprietary size-exclusion chromatography resin.

Quickly desalt protein and biological samples for manual or automated liquid chromatography (LC) with Thermo Scientific Zeba Spin and Micro Spin Desalting columns and plates (40K MWCO), which contain Zeba High-Performance Resin, a proprietary size-exclusion chromatography resin that offers excellent protein desalting and recovery in a centrifuge format.

Thermo Scientific Zeba Spin Desalting columns and plates (40K MWCO) are convenient, ready-to-use devices compatible with major automated liquid-chromatography systems or for manual syringe processing. The polypropylene columns have a 40K MWCO and contain a proprietary high-performance size-exclusion chromatography resin (Zeba High-Performance Resin) that offers excellent protein desalting and recovery in a centrifuge format. When using Zeba Micro Spin Desalting Columns, 40K MWCO, 75 μL, even very dilute (25 μg/mL) protein samples can be successfully processed to obtain greater than 95% retention (removal) of salts and other small molecules (<2000 MW), and good recovery of proteins and other macromolecules (>40,000 MW).

Zeba Spin Desalting Plates are polypropylene devices containing a proprietary high-performance size-exclusion resin for effective removal of salts and other small molecules (<2000 daltons) while providing excellent recovery for proteins >40,000 Da. They provide trouble-free desalting and buffer exchange for sample volumes ranging from 20 to 100 μL. This 96-well spin plate format enables more high-throughput processing.

The Zeba 96-well spin desalting plates require no resin dispensing or hydration and provide the same high protein recovery as spin columns with at least 95% removal of salts and other small molecules. One plate can process 96 small sample volumes (20 to 100 μL) in five minutes.

Benefits of Zeba Spin Desalting columns and plates include:
• High recovery—low-binding resin maximizes protein recovery
• Fast—no fraction screening or waiting for protein to emerge by gravity flow
• Economical—great performance at lower cost than other commercially available cartridges
• Easy-to-use—no cumbersome column preparation or equilibration
• Flexible—available in spin columns and filter spin plates for a range of needs

Available Formats
• Spin columns—75 μL, 0.5 mL, 2 mL, 5 mL, 10 mL columns
• 96-well spin plates—pre-dispensed 96-well filter plates, compatible with centrifugation for manual or automated purification

TRUSTED_SUSTAINABILITY

Specifications

Product Type Spin Desalting Column
Content And Storage Upon receipt store at 4°C.
Format Spin Column
Purification Target Buffer Exchange, Protein
Column Type Size-exclusion, Proprietary Resin
MWCO 40 kDa
Product Line Zeba
Quantity 5 Columns
Volume (Metric) 5 mL
Sample Volume (Metric) 300 to 2000 μL

Frequently Asked Questions (FAQs)

Besides precipitation with ammonium sulfate, what are some ways I can concentrate my protein sample?

You may remove excess solvent and smaller moieties by centrifugation through a microconcentrator. This will concentrate your protein sample.

(1) Choose microconcentrator tube (available from a variety of commercial suppliers) with a protein cutoff smaller than the molecular weight of the protein in the sample. Check our Pierce Protein Concentrators PES.

(2) Add 1 µL of 20% w/v SDS to a dry microconcentrator tube (if sample does not already contain SDS).

(3) Slowly add sample (a few microliters at a time) to membrane until membrane is completely wet. Centrifuge to near (but not complete) dryness.

(4) If intention is to desalt sample or buffer exchange: Add ~50 µL water to microconcentrator and spin until nearly dry. Repeat buffer exchange. Sample will remain on membrane. Check our Zeba desalting proteins.

(5) Using a new collection tube, invert membrane and spin at low speed (1000 x g) to elute protein from membrane. Add 2X SDS-Sample Buffer containing 10 mM DTT to membrane: vortex, invert and spin. Final volume should be ~20 µL.
Can you provide the shelf life for Zeba Spin Desalting columns?

Zeba Spin Desalting columns are covered under our general 1-year warranty and are guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf).

I used a Zeba desalting column, but it is not completely getting rid of glycerol and detergent from my sample. What should I do?

Glycerol and sugars add viscosity to a sample, while detergents create micelles, making the removal of each difficult. Although dialysis is a better choice for removing these components, Zeba Columns can be used by modifying the desalting protocol. Modifications include diluting the samples, processing smaller samples, and adding the sample slowly to the column allowing it to enter the gel and equilibrate into the pores before centrifugation.
After desalting with your Zeba desalting columns, I still have leftover salts in my sample. What should I do to get rid of all the salt?

You can either reduce the volume of sample processed in each column (the product manual indicates the volume range recommended for each column size) or run the flow-through from one column over a fresh column.
Can Zeba Columns be used for glycerol and detergent removal?

Glycerol and sugars add viscosity to a sample, while detergents create micelles, making the removal of each difficult. Although dialysis is a better choice for removing these components, Zeba Columns can be used by modifying the desalting protocol. Modifications include diluting the samples, processing smaller samples, and adding the sample slowly to the column allowing it to enter the gel and equilibrate into the pores before centrifugation.
Is my sample likely to become diluted following desalting with Zeba columns?

Centrifuged-based desalting results in minimal dilution. A slight dilution of the protein solution results if the optional stacker buffer is added that ensures maximum protein recovery.

When used properly, the volume recovered from the column equals the volume of sample (and buffer) added to the column.
Can Zeba columns be regenerated and stored after use?

Zeba columns are designed to be used once and discarded after use.
Can I remove DyLight, Alexa Fluor or other dyes using the desalting columns?

No, excess amounts of DyLight dyes (and any other planar molecule with multiple rings in their structure) “act” larger than their stated molecular weights in desalting columns. We do not recommend desalting, but refer researchers to our Pierce Dye Removal Columns (Cat. No. 22858) for this purpose.
Can I use Zeba columns for nucleic acids?

Yes, the 7K Zeba columns have good recovery of dsDNA ladder down to 10 bp.
Are Zeba columns compatible with salts/organics?

Yes, Zeba columns are compatible with most salts. The resin is stable to some organics. As organics may affect performance, we suggest using less than 10% organics.
How do your Zeba desalting products work?

A centrifuge is used to first remove the resin‘s void volume of liquid, followed by sample addition and centrifugation. After centrifugation, the macromolecules in the sample have moved through the column in approximately the same initial volume, but the small molecules have been forced into the pores of the resin and replaced by the buffer that was used to pre-equilibrate the gel-filtration matrix. Spin formats eliminate the need to wait for samples to emerge by gravity flow and require no chromatography system, allowing for multiple-sample processing simultaneously.

Note: The addition of larger volumes of buffer or longer centrifugation times than listed in the protocol will result in smaller molecules eventually emerging from the desalting column.
What is desalting?

Desalting is the process where porous particles or beads are used to separate molecules of different sizes (known as gel filtration or size exclusion). Small molecules enter into the pores in the resin, resulting in a longer path length through the desalting column when compared to large molecules which pass in the space between the beads This increased path length for molecules below the MWCO allows the separation of small and large molecules.

Our desalting resins are rated by what MWCO molecules can pass through. They also have a lower MWCO (1K or 2K) that can be effectively separated from larger molecules. Molecules in between these values cannot be effectively separated from larger or smaller molecules, given our protocols.
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