Oligonucleotides

Invitrogen™ Oligo(dT)12-18 Primer

Primer is suitable for use in first-strand cDNA synthesis with reverse transcriptase

Applied Biosystems™ Random Hexamers (50 µM)

Serve as primers for DNA synthesis by a DNA polymerase or reverse transcriptase

Thermo Scientific™ Random Hexamer Primer

Optimze cDNA synthesis with these random hexamer primers, oligo(dT)18 primers and anchored oligo dT primers.

Thermo Scientific™ Oligo(dT)18 Primer

Thermo Scientific™ Oligo(dT)18 Primer is a synthetic single-stranded 18-mer oligonucleotide with 5'- and 3'-hydroxyl ends.

Invitrogen™ Oligo(dT)20 Primer

Used for first-strand cDNA synthesis with reverse transcriptase at temperatures of ≥50°C

Invitrogen™ Random Primers

Truly random primers suitable for DNA synthesis using Klenow fragments with DNA templates or for cDNA synthesis using reverse transcriptase with mRNA templates

Invitrogen™ M13 Forward (-20)

Oligonucleotides complementary to a DNA template are necessary to prime DNA synthesis for sequencing reactions

Invitrogen™ Random Decamers (50 µM)

Provided at a stock concentration of 50μM, and functionally tested using the RETROscript™ Kit

Thermo Scientific™ Exo-Resistant Random Primer

Perform highly efficient random priming of DNA synthesis reactions with this mixture of single-stranded random oligonucleotides.

Invitrogen™ Anchored Oligo(dT)20 Primer

Primer mixture consisting of a string of 20 deoxythymidylic acid residues followed by dV (either dG, dA, or dC) and then by dN (dA, dT, dG, or dC)

Invitrogen™ T7 Promoter Primer

Primers for PCR amplification that complement many vectors

Thermo Scientific™ pJET1.2 Forward Sequencing Primer, 23-mer

Primers for sequencing of DNA fragments inserted into Eco32I site within the eco47IR gene of pJET1.2 and for colony screening by PCR.

Invitrogen™ pUC19 DNA (Sau3A I digested)

Ambion pUC 19 DNA is digested to completion with Sau3A I

Invitrogen™ Bio-11-UTP (75 mM)

Ideal for use as substrates as part of in vitro transcription reactions

Invitrogen™ Oligo (dT) Primer (50 µM)

Provided at a stock concentration of 50μM

Invitrogen™ 5-(3-Aminoallyl)-UTP (50 mM)

Ambion Modified nucleotides confer unique characteristics to the RNA molecules into which they are incorporated

Invitrogen™ 5-(3-Aminoallyl)-dUTP (50 mM)

When incorporated into DNA, 5-(3-aminoallyl)-dUTP provides a reactive group for addition of other chemical groups

Invitrogen™ M13 Reverse

Oligonucleotides complementary to a DNA template are necessary to prime DNA synthesis for sequencing reactions

Invitrogen™ Lambda Hind III dsDNA Markers

Ambion Lambda DNA is digested to completion with Hind III

Thermo Scientific™ M13/pUC Reverse Sequencing Primer (-26), 17-mer

Sequence DNA fragments inserted into the MCS of various pUC19-based cloning vectors with M13 pUC sequencing primers, single-stranded oligonucleotides.

Invitrogen™ Bio-11-UTP (10 mM)

Ideal for use as substrates as part of in vitro transcription reactions

Invitrogen™ Bio-16-UTP (10 mM)

Ideal for use as substrates as part of in vitro transcription reactions

Thermo Scientific™ T7 promoter Sequencing Primer, 20-mer

Perform sequencing of DNA fragments located downstream from the corresponding RNA polymerase promoter sequences in cloning vectors, such as pTZ19R, pTZ57R and pBluescript II.

Thermo Scientific™ SP6 promoter Sequencing Primer, 24-mer

Perform sequencing of DNA fragments located downstream from the corresponding RNA polymerase promoter sequences in cloning vectors, such as pTZ19R, pTZ57R and pBluescript II.

Invitrogen™ RNA Century™-Plus Marker Templates

Contain mixtures of linearized plasmids ready for use as templates during in vitro transcription reactions for synthesis of labeled RNA molecular size standards

Thermo Scientific™ SP6 promoter Sequencing Primer, 18-mer

Perform sequencing of DNA fragments located downstream from the corresponding RNA polymerase promoter sequences in cloning vectors, such as pTZ19R, pTZ57R and pBluescript II.

Applied Biosystems™ Oligo d(T)16 (50 µM)

Used for priming and reverse transcription of polyadenylated (poly A+) mRNA

Thermo Scientific™ M13/pUC Sequencing Primer (-40), 17-mer

Sequence DNA fragments inserted into the MCS of various pUC19 based cloning vectors with M13 pUC sequencing primers, single-stranded oligonucleotides.

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