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CD45 Rat anti-Mouse, FITC, Clone: 30-F11, BD
Rat Monoclonal Antibody
Marke: BD Biosciences 553079
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Beschreibung
The 30-F11 clone has been reported to react with all isoforms and both alloantigens of CD45, which is found on hematopoietic stem cells and all cells of hematopoietic origin, except erythrocytes. CD45 is a transmembrane glycoprotein which is expressed at high levels on the cell-surface, and its presence distinguishes leukocytes from non-hematopoietic cells. CD45 is a member of the Protein Tyrosine Phosphatase (PTP) family, where the intracellular carboxy-terminal region contains two PTP catalytic domains, and the extracellular region is highly variable due to alternative splicing of exons 4, 5, and 6 (designated as A, B, and C, respectively). CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction and the CD45 isoforms detected in the mouse are cell type-, maturation-, and activation state-specific. This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
Host Species: Rat
Clone: 30-F11
Isotype: IgG2b κ
Species Reactivity: Mouse
Immunogen: Mouse Thymus or Spleen
Flow Cytometry
Spezifikation
| CD45 | |
| Monoclonal | |
| 0.5mg/mL | |
| Ptprc; CD45R; LCA; Leukocyte common antigen; T200; B220; Ly-5; Lyt-4 | |
| Rat | |
| Affinity Purified | |
| RUO | |
| Primary | |
| Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |
| Flow Cytometry | |
| 30-F11 | |
| FITC | |
| Aqueous buffered solution containing ≤0.09% sodium azide. | |
| Mouse Thymus or Spleen | |
| 0.1 mg | |
| Immunology | |
| Mouse | |
| IgG2b κ |
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