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ETF1, Mouse, Clone: 2H4, Abnova™
Mouse monoclonal antibody raised against a partial recombinant ETF1.
Marke: Abnova H00002107-M02.100ug
Beschreibung
Termination of protein biosynthesis and release of the nascent polypeptide chain are signaled by the presence of an in-frame stop codon at the aminoacyl site of the ribosome. The process of translation termination is universal and is mediated by protein release factors (RFs) and GTP. A class 1 RF recognizes the stop codon and promotes the hydrolysis of the ester bond linking the polypeptide chain with the peptidyl site tRNA, a reaction catalyzed at the peptidyl transferase center of the ribosome. Class 2 RFs, which are not codon specific and do not recognize codons, stimulate class 1 RF activity and confer GTP dependency upon the process. In prokaryotes, both class 1 RFs, RF1 and RF2, recognize UAA; however, UAG and UGA are decoded specifically by RF1 and RF2, respectively. In eukaryotes, eRF1, or ETF1, the functional counterpart of RF1 and RF2, functions as an omnipotent RF, decoding all 3 stop codons (Frolova et al., 1994 [PubMed 7990965]).[supplied by OMIM
Sequence: TEEEKILYLTPEQEKDKSHFTDKETGQEHELIESMPLLEWFANNYKKFGATLEIVTDKSQEGSQFVKGFGGIGGILRYRVDFQGMEYQGGDDEFFDLDDYSpezifikation
| ETF1 | |
| Monoclonal | |
| Unconjugated | |
| PBS with no preservative; pH 7.4 | |
| NM_004730 | |
| ETF1 | |
| ETF1 (NP_004721.1, 338 a.a. ∼ 437 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. | |
| 100 μg | |
| Primary | |
| Human | |
| Antibody | |
| IgG2b κ |
| ELISA, Western Blot | |
| 2H4 | |
| Mouse monoclonal antibody raised against a partial recombinant ETF1. | |
| ETF1 | |
| D5S1995/ERF/ERF1/MGC111066/RF1/SUP45L1/TB3-1 | |
| Mouse | |
| Affinity chromatography | |
| RUO | |
| 2107 | |
| Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. | |
| Liquid |
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