IKKγ (pS376) Mouse anti-Human, PE, Clone: N19-39, BD

Beschreibung

The N19-39 monoclonal antibody specifically binds to the IKK-gamma protein phosphorylated at the Ser376 site, ie, IKKγ (pS376). IKKγ is also known an NEMO (NF-κ-B essential modifier) and is encoded by the IKBKG (inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma) gene. The nuclear transcription factor κ-B, NF-κB, is controlled by interaction with an inhibitory subunit, IκB, which restricts NF-κB to the cytoplasm. Following stimulation by various cytokines or other stimuli, IκB becomes degraded and NF-κB is released to the nucleus. The release of IκB from NF-κB is thought to be a critical point in the activation of NF-κB signal pathways. Activated NF-κB regulates genes involved in various pathways including inflammation, immunity, and cell survival signaling pathways. A group of proteins form an NF-κB regulatory complex, or signalsome. Two members of this complex are a pair of closely related serine/threonine kinases, IKKα and IKKβ (also called IKK-1 and IKK-2), which phosphorylate critical residues of IκB, thus targeting it for subsequent degradation. The IKK complex contains similar amounts of IKKα, IKKβ as well as two other polypeptides, which are differentially processed forms of a third subunit, IKKγ. IKKα and IKKβ become activated following phosphorylation by upstream kinases, including NF-κB-inducing kinase (NIK)4 and MEKK1. IKKβ has been shown to phosphorylate IKKγ at serine 376 in response to signaling through the TNF receptor or the Tax oncoprotein of human T-cell leukemia virus type 1. IKKγ has an essential regulatory role in the IKK complex and is required for the activation of the NF-κB pathway in response to multiple cellular stimuli. IKKγ isoforms migrate as a doublet of ∽50-55kDa upon Western blot analysis.

Host Species: Mouse
Clone: N19-39
Isotype: IgG1, κ
Species Reactivity: Human
Immunogen: Phosphorylated Human IKKγ Peptide

Flow Cytometry