Rap2 Mouse, Unlabeled, Clone: 12, BD

Rap2 is a member of the Ras superfamily of low molecular weight GTP/GDP binding proteins. The Rap proteins are 50% homologous in sequence to p21ras. Like Ras, the Rap proteins cycle between a GDP-bound inactive form and a GTP-bound active form. Conversion between these two forms is regulated by Rap-GTPase activating protein (Rap-GAP) and a Rap-GDP dissociation stimulator (GDS). Since Ras and Rap have the same amino acid sequence in their putative effector domain (aa. 32-40), it seems likely that they perform either similar or antagonistic functions. Rap2 proteins are about 60% identical to Rap1 proteins and Rap2A and Rap2B show 90% amino acid identity, differing mainly at the carboxy-terminus. Unlike Rap1, the Rap2 proteins cannot compete with Ras for interaction with Ras-GAP, nor are they substrates for PKA. It follows that the intrinsic GTPase activity of Rap2A is not stimulated by Ras-GAP; however a distinct activator (Rap-GAP) has been identified. Both Rap2 proteins show posttranslational modifications: Rap2B is geranylgeranylated and Rap2A is the first non-Ras member of the Ras superfamily observed to be farnesylated.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.