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Applied Biosystems™ TaqMan™ Reverse Transcription Reagents
Beschreibung
Beinhaltet
MultiScribe™ Reverse Transcriptase (100μL at 50U/μL)
TaqMan Reverse Transcription Reagents provide the necessary reagents for reverse transcriptase-PCR (RT-PCR). Random hexamers, oligo (dT)16, and reverse primers are included for cDNA synthesis flexibility. These reagents are designed for reverse transcription and PCR amplification of a specific target RNA from either total RNA or mRNA. The reagents in this kit facilitate two-step RT-PCR, which is performed in two separate reactions and is useful when detecting multiple transcripts from a single cDNA reaction or when storing a portion of the cDNA.
Advantages of TaqMan Reverse Transcription Reagents
- Contains MultiScribe Reverse Transcriptase, a recombinant Moloney murine leukemia virus (MMLV) reverse transcriptase
- Includes random hexamers and oligo (dT)16 for flexibility
- Individual components provide versatility in assay setup
- Compatible with two-step RT-PCR reactions
Using TaqMan Reverse Transcription Reagents
- TaqMan Reverse Transcription Reagents provide all the necessary components for reverse transcription of RNA to cDNA
- For two-step RT-PCR, random hexamers, oligo (dT)16, and sequence-specific reverse primers can all be used
- The choice of primers for reverse transcription is best made after experimentally evaluating all three priming systems
Spezifikation
Spezifikation
| Concentration | 50 U/μL |
| Content And Storage | • MultiScribe Reverse Transcriptase, 100 μL (50 U/μL) |
| Format | Kit |
| Technique | Reverse Transcription |
| Optimal Reaction Temperature | 42°C |
| Quantity | 200 Reactions |
| Reverse Transcriptase | MultiScribe |
| Ribonuclease H Activity | Yes |
| Shipping Condition | Dry Ice |
| For Use With (Application) | Real Time PCR (qPCR) |
| Mehr anzeigen |
For Research Use Only. Not for use in diagnostic procedures.
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