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  7. Applied Biosystems™ TaqMan™ Reverse Transcription Reagents 

Applied Biosystems™ TaqMan™ Reverse Transcription Reagents

Product Code. 10117254
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No. of Reactions:
200 Reactions at 10 μL
2000 Reactions at 10 μL
Unit Size:
1 set
Each
Product Code. No. of Reactions unitSize
10117254 200 Reactions at 10 μL Each
10279624 2000 Reactions at 10 μL 1 set
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For Research Use Only. All usage must comply with product instructions.

Product Code. 10117254

Brand: Applied Biosystems™ N8080234

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721.58 EUR valid until 2026-02-28
BEST PRICE on Promo! Use promo code "28033" to get your promotional price.
This item is not returnable. View return policy

For Research Use Only. All usage must comply with product instructions.

Description

Includes

MultiScribe™ Reverse Transcriptase (100μL at 50U/μL)

TaqMan Reverse Transcription Reagents provide the necessary reagents for reverse transcriptase-PCR (RT-PCR). Random hexamers, oligo (dT)16, and reverse primers are included for cDNA synthesis flexibility.

TaqMan Reverse Transcription Reagents provide the necessary reagents for reverse transcriptase-PCR (RT-PCR). Random hexamers, oligo (dT)16, and reverse primers are included for cDNA synthesis flexibility. These reagents are designed for reverse transcription and PCR amplification of a specific target RNA from either total RNA or mRNA. The reagents in this kit facilitate two-step RT-PCR, which is performed in two separate reactions and is useful when detecting multiple transcripts from a single cDNA reaction or when storing a portion of the cDNA.

Advantages of TaqMan Reverse Transcription Reagents

  • Contains MultiScribe Reverse Transcriptase, a recombinant Moloney murine leukemia virus (MMLV) reverse transcriptase
  • Includes random hexamers and oligo (dT)16 for flexibility
  • Individual components provide versatility in assay setup
  • Compatible with two-step RT-PCR reactions

Using TaqMan Reverse Transcription Reagents

  • TaqMan Reverse Transcription Reagents provide all the necessary components for reverse transcription of RNA to cDNA
  • For two-step RT-PCR, random hexamers, oligo (dT)16, and sequence-specific reverse primers can all be used
  • The choice of primers for reverse transcription is best made after experimentally evaluating all three priming systems
TRUSTED_SUSTAINABILITY

Specifications

Concentration 50 U/μL
Content And Storage

• MultiScribe Reverse Transcriptase, 100 μL (50 U/μL)
• RNase Inhibitor, 100 μL (20 U/μL)
• dNTP Mixture, 1 mL (2.5 mM each dNTP)
• Oligo (dT)16, 100 μL (50 μM)
• Random Hexamers, 100 μL (50 μM)
• 10X RT Buffer, 1.5 mL
• MgCl2 Solution, 1.5 mL

Store at –25°C to –15°C.
Format Kit
Technique Reverse Transcription
Optimal Reaction Temperature 42°C
Quantity 200 Reactions
Reverse Transcriptase MultiScribe
Ribonuclease H Activity Yes
Shipping Condition Dry Ice
For Use With (Application) Real Time PCR (qPCR)
Final Product Type First-Strand cDNA
No. of Reactions 200 Reactions at 10 μL
Reaction Format Separate components
Reagent Type Reverse Transcription
Size (Final Product) Up to 5 kb
Starting Material RNA
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For Research Use Only. Not for use in diagnostic procedures.

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