Invitrogen™ ATF6 Recombinant Rabbit Monoclonal Antibody (PSH06-59)

Beschreibung

Positive Control: HeLa cell lysate, A431 cell lysate, HUVEC cell lysate, 293T cell lysate, HepG2 cell lysate, RAW264.7 cell lysate, PC-12 cell lysate, Mouse pancreas tissue lysate, Mouse kidney tissue lysate, Rat pancreas tissue lysate, Rat kidney tissue lysate, rat pancreas tissue, rat kidney tissue. Tissue Specificity: Low tissue specificity. Subcellular Location: Endoplasmic reticulum membrane, Golgi apparatus membrane. Sequence Similarities: 83% Mouse/Rat Predicted band size: 75 kDa.

ATF6 is a transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. It binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. ATF6 could also be involved in activation of transcription by the serum response factor. ATF6 exists as a homodimer and heterodimer with ATF6 beta. The dimer interacts with the nuclear transcription factor Y (NF-Y) trimer through direct binding to NF-Y subunit C (NF-YC). It Interacts also with the transcription factors GTF2I, YY1 and SRF. Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus. The basic domain of ATF6 functions as a nuclear localization signal and the basic leucine-zipper domain is sufficient for association with the NF-Y trimer and binding to ERSE. During the unfolded protein response an approximately 50 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases. ATF6 is N-glycosylated, phosphorylated in vitro by MAPK14/P38MAPK and belongs to the bZIP family.