Invitrogen™ Bax Monoclonal Antibody (6A7)

Beschreibung

MA5-14003 targets Bax in Western blot, FACS, immunohistochemistry (paraffin) and ICC/IF applications and shows reactivity with Human, mouse, and Rat samples. This antibody is not recommended for Jurkat cells in Western blot applications. The MA5-14003 immunogen is a Synthetic peptide, aa 12-24 (Cys-GPTSSEQIMKTGA), of human Bax protein. This amino acid sequence is shared by human, mouse and rat Bax protein.

BAX is a members of the Bcl-2 Family and plays an important role in regulation of apoptosis. Whereas Bcl-2 is commonly regarded as an anti-apoptotic protein, BAX is considered to have a pro-apoptotic function. Regulation of apoptosis is supposed to involve both homo- and heterodimerization of different isoforms of BAX and Bcl-2. The Bax gene encodes different isoforms including Bax alpha (21 kDa) and Bax beta (24 kDa), whereas both isoforms contain the BH1, BH2 and BH3 domains, Bax beta has a unique carboxyl terminus and does not contain a hydrophobic transmembrane domain. Bcl-2 is also expressed in different Isoforms. Bcl-2 beta differs in the 3' UTR and coding region compared to variant alpha. Bcl-2 beta is shorter (22 kDa) and has a distinct C-terminus compared to Bcl-2 alpha (26 kDa). BAX is a member of the BCL-2 family of proteins, which function as regulators of apoptosis. Overexpression of BAX functions to promote cell death. BAX can form homodimers and is also able to heterodimerize with other BCL-2 related proteins.