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Invitrogen™ Bovine IL-4 Recombinant Protein
Beschreibung
For dilution, use a buffer containing carrier protein, e.g. PBS with 1% BSA or a neutral media containing 10% FCS and freeze in aliquots at -70°C. Activity: The biological activity of recombinant bovine IL-4 was determined by measuring the proliferation of the factor-dependent human T-helper cell line, TF-1 (Kitamura, T. et al. (1989) J. Cell Physiol. 140:323). The ED50 (or 1 unit of activity) for this effect in our laboratories typically falls into the range of 0.44 - 0.67 ng/mL. Expression: Mature bovine IL-4 was expressed in Pichia pastoris, X-33. GenBank™ Accession # P30367, amino acids 25 - 135. For protein verification, the expressed protein was N-terminal sequenced and begins with the following 10 amino acids: HKCDITLAEI. Reconstitute with 0.5 mL of distilled water.
Spezifikation
Spezifikation
| Zugriffsnummer | P30367 |
| Zur Verwendung mit (Anwendung) | ELISA, Immunoprecipitation, Western Blot |
| Zusammensetzung | PBS with no preservative |
| Gen-ID (Entrez) | 280824 |
| Molekulargewicht | 12.6 kDa |
| Name | Bovine IL-4 |
| Menge | 20 μg |
| Quelle | Yeast |
| Kennzeichnung | RUO |
| Gen-Alias | B cell growth factor 1; B_cell stimulatory factor 1; B-cell growth factor 1; B-cell IgG differentiation factor; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; cytokine; H-IL-4; IGG1 induction factor; IL2; IL4; Il-4; Il4e12; ILN; Interleukin; interleukin 4; interleukin 4 variant 2; interleukin 4 variant IL-4 delta2; interleukin 4 variant IL-4 delta3; interleukin 4 variant IL-4 int2A; interleukin 4 variant IL-4 int3A; interleukin 4 variant IL-4 int3B; Interleukin4; interleukin-4; interleukin-4 precursor; lymphocyte stimulatory factor 1; MGC79402; M-IL-4; Pitrakinra |
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