Thermo Scientific™ CaptureSelect™ FcXP RoboColumn™ Pre-packed columns

CaptureSelect FcXP affinity matrix recognizes the CH3 domain of all human IgG subclasses (IgG1, 2, 3, and 4), enabling purification of all IgG subclasses and Fc fusion proteins directly from complex source materials in a robust and efficient purification process with excellent purity obtained in a single step.

Features of tFcXP affinity matrix include:

• Mild elution, making it suitable for Fc fusion proteins
• One-step purification from crude material with high purity and yield
• Human specific, no binding to bovine antibodies
• Excellent scalability
• Non–animal-derived

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Characteristics

• Column size: 200 μL, 1 strip, 8 columns (8-row)
• Column dimension: 5-mm diameter, 10-mm bed height
• Matrix: agarose-based, epoxide-activated
• Average particle size: 65 ± 10 μm
• Ligand: CaptureSelect FcXP affinity ligand
• Ligand coupling: epoxide coupling
• Binding capacity: 40-50 g IgG/L resin depending on flow rate, column height, and contact time
• Elution conditions: 20 mM citric acid or acetic acid, pH 3–4
• Formulation buffer: 20% (v/v) ethanol
• Operating pressure: 2 bar (maximum)
• Flow rate: 16–150 cm/hr