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Beschreibung
CaptureSelect HRP Anti-AAV9 Conjugate includes a 13-kDa recombinant single domain antibody fragment (VHH affinity ligand) that specifically binds adeno-associated virus particles of serotype 9 (AAV9).
CaptureSelect HRP Anti-AAV9 Conjugate can be combined with CaptureSelect Biotin Anti-AAV9 Conjugate (Cat. No. 7103332100) in enzyme-linked immunoassays to quantify the total number of AAV9 capsids. Sandwich ELISA set-ups can be designed using the biotin anti-AAV9 conjugate as a capture reagent immobilized on a streptavidin coated surface. HRP anti-AAV9 conjugate is then used for detection of bound AAV9 capsids.
For assay development to measure total capsids for other AAV serotypes, we recommend CaptureSelect Biotin Anti-AAVX Conjugate (Cat. No. 7103522100) and CaptureSelect HRP Anti-AAVX Conjugate (Cat. No. 7303522100).
Product details
- Format type: HRP, liquid
- Buffer: PBS, 50% (v/v) glycerol
- Storage: in freezer (-5 to -30°C)
- Concentration: 0.5 mg/mL
- Volume: 0.1 mL
- Recommended working dilution for sandwich ELISA: 1:5000 to 1:10,000 (the optimal working dilution should be determined empirically for each application)
Free of animal components
CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).
Spezifikation
Spezifikation
| Konzentration | 0.5 mg/mL |
| Zusammensetzung | Liquid, in PBS (7.4), 50% glycerol |
| Inhalt und Lagerung | Store in freezer (-5°C to -30°C). |
| Form | Flüssig |
| Produktlinie | CaptureSelect |
| Menge | 100 μl |
| Zur Verwendung mit (Anwendung) | Quantitation of AAV9 Capsids |
| Typ | Conjugate |
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