Invitrogen™ CD11b Monoclonal Antibody (M1/70), eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Description: The M1/70 monoclonal antibody reacts with mouse CD11b, the 165-170 kDa integrin alphaM. CD11b non-covalently associates with CD18 to form alphaMbeta2 integrin (Mac-1) and binds to CD54 (ICAM-1), C3bi, and fibrinogen. Mac-1 is expressed by macrophages, NK cells, granulocytes, activated lymphocytes and mouse B-1 cells in the peritoneal cavity. M1/70 is also cross-reactive to human CD11b, and can be used for the detection of this antigen on human peripheral blood monocytes, granulocytes, and a subset of NK cells. Through interactions with its ligands, CD11b participates in adhesive cell interactions. Applications Reported: The M1/70 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue. The M1/70 clone is not recommended for immunohistochemical staining of formalin-fixed paraffin embedded tissue. M1/70 has also been reported in in vitro blocking of CD11b function. (Please use Functional Grade purified M1/70, cat. 16-0112, in functional assays.). Applications Tested: The M1/70 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.