CD158f Mouse anti-Human, Brilliant Violet 421, Clone: UP-R1, BD

Mouse Monoclonal Antibody

The UP-R1 monoclonal antibody specifically recognizes CD158f which is also known as KIR2DL5. CD158f is a ñ60 kDa single-pass type I transmembrane glycoprotein that belongs to the KIR (Killer cell Ig-like receptors) family within the Ig gene superfamily. CD158f is encoded by two gene paralogs, KIR2DL5A and KIR2DL5B /that show greater than 99% identity in their coding sequences. /CD158f molecules contain two extracellular C2-type Ig-like domains and a long cytoplasmic domain containing one canonical immunoreceptor tyrosine-based inhibitory motif (ITIM) and another atypical ITIM sequence. Although the exact ligands for CD158f have not yet been identified, the inhibitory function of UP-R1 antibody-crosslinked CD158f was shown to primarily depend upon a Src homology region 2-containing protein tyrosine phosphatase-2 (SHP-2) -dependent pathway. CD158f is highly polymorphic and is expressed on subsets of NK cells and T lymphocytes by some individuals. UP-R1 recognizes cell surface CD158f encoded by the most commonly expressed allele, KIR2DL5A*001 . The UP-R1 antibody has been predicted to recognize CD158f molecules encoded by other transcriptionally-active KIR2DL5 alleles, A*012 and B*00602