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Invitrogen™ CD170 (Siglec F) Monoclonal Antibody (1RNM44N), NovaFluor™ Blue 800, eBioscience™
Beschreibung
Description: This 1RNM44N monoclonal antibody recognizes mouse CD170, also known as Siglec F. Mouse Siglec F is a functional ortholog of human Siglec 8, however, unlike human Siglec 8, mouse Siglec F is not expressed on mast cells. This 1RNM44N antibody will recognize a formaldehyde-fixed epitope. This product contains 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer. Applications Tested: This 1RNM44N antibody has been tested by flow cytometric analysis of mouse thioglycolate-elicited peritoneal exudate cells. This can be used at less than or equal to 0.06 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest Master mixes. Whole Blood compatibility: When utilizing whole blood (as opposed to density-gradient-purified PBMC), we recommend lysing red blood cells in bulk prior to staining with NovaFluor conjugates.
Spezifikation
Spezifikation
| Antigen | CD170 (Siglec F) |
| Anwendungen | Flow Cytometry |
| Klassifikation | Monoclonal |
| Klon | 1RNM44N |
| Konzentration | 0.1 mg/mL |
| Konjugat | NovaFluor Blue 800 |
| Zusammensetzung | PBS with BSA and 0.09% sodium azide; pH 7.2 |
| Gen | Siglecf |
| Gen-Zugriffsnummer | Q920G3 |
| Gen-Alias | CD170; CD33 antigen-like 2; CD33L2; mSiglec-F; OB-binding protein 2; OBBP2; OB-BP2; Obesity-binding protein 2; sialic acid binding Ig like lectin 5; sialic acid binding Ig-like lectin 5; sialic acid binding Ig-like lectin F; sialic acid-binding Ig-like lectin 5; Sialic acid-binding Ig-like lectin F; sialic acid-binding immunoglobulin-like lectin 5; sialic acid-binding lectin Siglec-F; Siglec; SIGLEC5; siglec-5; Siglecf; Siglec-F |
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