CD74 Mouse anti-Human, Brilliant Violet 650, Clone: LN2 (also known as LN-2), BD Optibuild™

Beschreibung

The LN2 monoclonal antibody specifically recognizes CD74, the cell surface MHC class II-associated invariant chain molecule (41, 35, 33 kDa). CD74 is moderately expressed on B cells, and weakly on monocytes and macrophages. Other reactive cells include Langerhans cells, dendritic cells, a subset of activated T cells and thymic epithelium. The LN2 monoclonal antibody labels a nuclear membrane protein of follicular center and mantle zone B cells and a cytoplasmic protein of cells which bind HLA class II antibodies. CD74 is postulated to play a role in antigen processing and presentation through intracellular transport and expression of class II molecules on the cell surface. This antibody is suitable for staining formalin-fixed, paraffin-embedded tissue sections without citrate pretreatment.

The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 650nm. BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination. BV650 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 650 nm. Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. BV650 will have moderate spillover into the BD Horizon™ BV711 detector.