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CHO/dhFr- (Chinese hamster ovary) Whole cell lysate, Non-denatured; Abnova

Artikelnummer. 16069635
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Menge:
200 μg
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200 Mikrogramm
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Product Code. 16069635

missing translation for 'mfr': Abnova L057V2.200ug

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Description

Western Blotting, Immunoprecipitation

Specifications

Zur Verwendung mit (Anwendung) Immunoprecipitation, Western Blot
Tissue Ovary
Beschreibung Whole cell lysate (non-denatured)
Lysepuffer Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Vorbereitungsmethode Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Qualitätskontrollen 12.5% SDS-PAGE Stained with Coomassie Blue.
Menge 200 μg
Lagerungspuffer In modified RIPA Lysis Buffer.
Wirtsspezies Hamster
Inhalt und Lagerung Lagerung bei -80 °C. Aliquot, um wiederholtes Einfrieren und Autauen zu vermeiden.
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