Invitrogen™ Collagen X Monoclonal Antibody (X53), eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Description: The monoclonal antibody X53 recognizes human, mouse, porcine, guinea pig, and rat Collagen Type X. X53 does not cross-react with collagen types I, II, III, IV, or VI. Collagen Type X is a homotrimeric short chain collagen. Collagen Type X has limited expression in normal adult tissues, primarily being expressed during bone growth and development. Expression is localized to the peri- and extracellular matrix of hypertrophic chondrocytes during endochondral ossification and also plays a role in cartilage mineralization. Collagen Type X is elevated in many tumor types with expression being localized in the vasculature. Mutations to the gene COL10A1, which encodes human Collagen Type X, is associated with Schmid type metaphyseal chondrodysplasia. Applications Reported: This X53 antibody has been reported for use in microscopy, immunohistochemical staining, ELISA, and immunocytochemistry. Applications Tested: This X53 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at less than or equal to 10 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.