Invitrogen™ Cutaneous Lymphocyte Antigen (CLA) Monoclonal Antibody (HECA-452), eFluor™ 660, eBioscience™

Rat Monoclonal Antibody

Description: This HECA-452 monoclonal antibody reacts with human Cutaneous Lymphocyte Antigen (CLA). This cell surface glycoprotein is most highly expressed on T cells in the skin. Nevertheless, CLA can also be detected on some peripheral T and NK cells, as well as monocytes, neutrophils and CD1a+ dendritic cells. Studies have determined that this antibody recognizes sialofucosylated glycans and sialyl Lewis x (sLex). CLA, which binds E-selectin and endothelial cell-leukocyte adhesion molecule 1 (ELAM-1), is involved in T lymphocyte infiltration and adhesion at cutaneous sites of inflammation. The HECA-452 antibody has been reported to inhibit sLex binding to E-selectin; however, this property is still under debate in the literature. Applications Reported: This HECA-452 antibody has been reported for use in flow cytometric analysis, western blotting, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. Applications Tested: This HECA-452 antibody has been tested by flow cytometric analysis on normal human peripheral blood cells. This HECA-452 antibody has also been tested by immunhistochemistry on FFPE human tissue and can be used at less than or equal to 20 μg/mL.The antibody performs optimally with low pH antigen retrieval but high pH antigen retrieval can also be used. eFluor™ 660 is a replacement for Alexa Fluor™ 647. eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that you...