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Invitrogen™ Cytochrome C Monoclonal Antibody (37BA11)
Mouse Monoclonal Antibody
Marke: Invitrogen™ 456100
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Beschreibung
45-6100 was used successfully in the western blot analysis of cytochrome C using human breast cancer cell line lysates. Positive control: Isolated mitochondria from Human, Bovine, Rat and Mouse heart; Human fibroblasts; HeLa cells. WB: Predicted molecular weight: 14 kDa. ICC/IF: Heat induced antigen retrieval in 0.1 M Tris-HCl, 5% urea, pH 9.5 for 5 min at 95°C improves signal.
Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in oxidative phosphorylation and the production of cellular ATP. Release of cytochrome C from the mitochondria to the cytosol is triggered by apoptotic stimuli. In the cytoplasm, the cytochrome C binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis.
Spezifikation
| Cytochrome C | |
| Monoclonal | |
| 1 mg/mL | |
| HEPES buffered saline with 0.02% sodium azide | |
| P62894, P62897, P62898, P99999 | |
| Cycs | |
| Bovine heart Cytochrome C. | |
| 100 μL | |
| Primary | |
| Human, Mouse, Rat, Bovine, C. elegans | |
| Antibody | |
| IgG2a κ |
| Flow Cytometry, Western Blot, Immunocytochemistry | |
| 37BA11 | |
| Unconjugated | |
| Cycs | |
| CG17903; CG17903-PA; CG17903-PB; CRF; CYC; CYC2; Cycs; CYCSA; Cyct-c-p; Cyt c; cyt c-p; cyt.c; CytC; Cyt-c; CYTC2; Cyt-c2; CytC-2; cyt-c-p; cytC-p; Cyt-c-p-PA; Cyt-c-p-PB; Cytochrome c; cytochrome c DC4; Cytochrome c proximal; Cytochrome C, expressed in somatic tissues; cytochrome c, somatic; Cytochrome c-2; cytochrome c-like; cytochrome c-like protein; Cytochrome c-proximal; cytochrome-c-p; Cytochrome-c-proximal; DC4; dCyt.C-p; DMc01; Dmel\CG17903; Dmel_CG17903; hc1; HCS; l(2)k13905; lethal (2) k13905; LOC100341175; LOC100357840; MGC128356 protein; pDMc01; RH17228p; THC4 | |
| Mouse | |
| IgG fraction | |
| RUO | |
| 13063, 25309, 510767, 54205 | |
| -20°C, Avoid Freeze/Thaw Cycles | |
| Liquid |
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