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Cytokeratin, pan Antibody (AE-1/AE-3), Novus Biologicals™

Artikelnummer. 18463492 Alle ansehen Bio Techne Produkte
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Menge:
20 ug
Packungsgröße:
0.10 Milligramm
20 Mikrogramm
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18463492 20 ug 20 Mikrogramm
18779173 - 0.10 Milligramm
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Artikelnummer. 18463492 Lieferant Novus Biologicals Lieferanten-Nr. NBP22942920ug

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Beschreibung

Mouse Monoclonal Antibody has been used in 28 publications

Cytokeratin, pan Monoclonal specifically detects Cytokeratin, pan in Human, Mouse, Rat, Bovine, Canine, Chicken, Monkey, Rabbit, Reptile, Zebrafish samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow (Intracellular), CyTOF-reported, Single Cell Western, Dual RNAscope ISH-IHC.
TRUSTED_SUSTAINABILITY

Spezifikation

Antigen pan Cytokeratin
Anwendungen Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), CyTOF, Western Blot, In Situ Hybridization (ISH)
Klassifikation Monoclonal
Klon AE-1/AE-3
Konzentration 0.2 mg/ml
Konjugat Unconjugated
Verdünnung Western Blot 1 - 2 ug/mL, Flow Cytometry 1 - 2 ug/ million cells, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1 - 2 ug/mL, Immunohistochemistry-Paraffin 0.25 - 1.0 ug/mL, Immunohistochemistry-Frozen 0.25 - 1.0 ug/mL, Flow (Intracellular), CyTOF-reported, Single Cell Western 1:10, Dual RNAscope ISH-IHC
Gen-Zugriffsnummer Q7Z794, Q01546
Gensymbole KRT1
Wirtsspezies Mouse
Immunogen This Cytokeratin, pan Antibody (AE-1/AE-3) was developed against total keratin isolated from human epidermal callus was used as immunogen to generate the pan cytokeratin antibodies AE1 + AE3 (Woodcock-Mitchell, 1982).
Reinigungsverfahren Protein A or G purified
Menge 20 ug
Regulatorischer Status RUO
Forschungsgebiet Apoptosis, Cancer, Cell Biology, Cellular Markers, Signal Transduction
Primär oder sekundär Primary
Gen-ID (Entrez) 3848
Testspezifität Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8); 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE-1/AE-3 is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas.
Zielspezies Human, Mouse, Rat, Bovine, Canine, Chicken, Primate, Rabbit, Reptile, Zebrafish
Inhalt und Lagerung Store at 4C.
Form Purified
Isotype IgG1 κ
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