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AcroBiosystems CytoPak GMP Human IL-2 Protein

Artikelnummer. 31452286
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Menge:
15 x 106 IU
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Artikelnummer. 31452286

Marke: AcroBiosystems GMPL02H14GB0115×10^6IU

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Beschreibung

Recombinant Protein

Interleukin-2 (IL-2) is an interleukin, a type of cytokine immune system signaling molecule, which is a leukocytotrophic hormone that is instrumental in the body's natural response to microbial infection and in discriminating between foreign (non-self) and self. IL-2 mediates its effects by binding to IL-2 receptors, which are expressed by lymphocytes, the cells that are responsible for immunity. Mature human IL-2 shares 56% and 66% aa sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit crossspecies activity. The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes. IL-2 is also necessary during T cell development in the thymus for the maturation of a unique subset of T cells that are termed regulatory T cells (T-regs). After exiting from the thymus, T-Regs function to prevent other T cells from recognizing and reacting against self antigens, which could result in autoimmunity. T-Regs do so by preventing the responding cells from producing IL-2. Thus, IL-2 is required to discriminate between self and non-self, another one of the unique characteristics of the immune system.

Spezifikation

Zugriffsnummer Uniprot
Charakterisierung IL-2 Structure This protein carries no tag. The protein has a calculated MW of 15.4 kDa. The protein migrates as 16 kDa±2 kDa when calibrated against under reducing (R) condition (SDS-PAGE).
Format Liquid
Zusammensetzung Supplied as 0.2 μm filtered solution in phosphate with protectants. Contact us for customized product form or formulation.
Qualitätskontrollen <0.5 ng/μg of protein tested by ELISA.∣<0.1 ng/μg of protein tested by qPCR.∣Less than 5.0 EU/mL, tested by the LAL method in compliance with USP 85 and Ph. Eur. 2.6.14.∣Sterility testing was performed using the membrane filtration method in compliance with USP &CM and Ph. Eur. 2.6.1.∣Negative
Menge 15 x 106 IU
Quelle CytoPak GMP Human IL-2 Protein (GMP-L02H14GB01) is expressed from E. coli cells. It contains AA Ala 21 - Thr 153 (Accession # ). Predicted N-terminus: Met
Biologische Aktivität CytoPak GMP Human IL-2 Protein (Cat. No. GMP-L02H14GB01) stimulates proliferation of CTLL-2 cells. The specific activity of CytoPak GMP Human IL-2 Protein is ≥1.20 x 10^7 IU/mg, which is calibrated against human Interleukin-2 China National Standard (NIFDC code: 270008) (QC tested). China National Institutes for Food and Drug Control (NIFDC) Standard was prepared and calibrated against human IL-2 WHO International Standard (NIBSC code: 86/500) by NIFDC.∣Human PBMCs were cultured with lyophilized GMP Human IL-2 Protein (ACROBiosystems, Cat. No. GMP-L02H14) or CytoPak GMP Human IL-2 Protein (ACROBiosystems, Cat. No. GMP-L02H14GB01) with CelThreaTM GMP T Cell Expansion Medium (ACROBiosystems) for two weeks. No significant differences were observed in cell viability or proliferation between lyophilized and CytoPak IL-2. Data is the average of 3 independent experiments, error bars ±SD.∣Human PBMCs were cultured with lyophilized GMP Human IL-2 Protein (ACROBiosystems, Cat. No. GMP-L02H14) or CytoPak GMP Human IL-2 Protein (ACROBiosystems, Cat. No. GMP-L02H14GB01) with CelThreaTM GMP T Cell Expansion Medium (ACROBiosystems) for two weeks. No significant differences were observed in the ratio of CD3+ or CD3+ CD4/CD8 positive cells between lyophilized and CytoPak IL-2. Data is the average of 3 independent experiments, error bars ±SD.∣The Cell based assay shows that CytoPak GMP Human IL-2 Protein (Cat. No. GMP-L02H14GB01) is stable at 25°C for 65 hours.∣The Cell based assay shows that CytoPak GMP Human IL-2 Protein (Cat. No. GMP-L02H14GB01) is stable after freezing and thawing 3 times.∣Consistent specific activity (IU/mg) is maintained across lyophilized IL-2 (Cat. No. GMP-L02H14), liquid IL-2 (Cat. No. GMP-L02H14F002), and Cytopak IL-2 (Cat. No. GMP-L02H14GB01).
Spezies Human
Sterilität Sterile
Inhalt und Lagerung -70°C for 24 months; Can be stored up to 1 month at -20°C; Can be stored up to 1 week at 2°C to 8°C. Upon receipt, store it immediately at -70°C or lower for long term storage.
Endotoxin-Gehalt Less than 5.0 EU/mL, tested by the LAL method in compliance with USP 85 and Ph. Eur. 2.6.14.
Versandbedingung This product is supplied and shipped with dry ice, please inquire the shipping cost.
Reinheits- oder Qualitätsgrad 0.95
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