Invitrogen™ E-cadherin Monoclonal Antibody (ECCD-2)

Rat Monoclonal Antibody

Source: Monoclonal antibody was obtained by fusing the P3-X63-Ag8 mouse myeloma cell line with spleen cells of Donryu rat after immunization with mouse teratocarcinoma and mouse liver E-cadherin fragment. Specificity: This antibody specifically reacts with mouse E-cadherin. This antibody does not inhibit E-cadherin-dependent cell-cell contact. Cross reactivity: This antibody reacts with human and dog E-cadherin. This antibody does not react with bovine or chicken E-cadherin. Western blot can be performed under reducing or non-reducing conditions. Reconstitution and dilution: dissolve the lyophilized antibody in 50 μl of distilled water to a final concentration 2.0 mg/mL. This solution can be used as a stock solution. If dilution is necessary for your application, dilute the stock solution in 500 μL or more of the following dilution solution: 20mM TBS (pH 7.5), 10mM CaCl2, 1.0% BSA, 0.1% NaN3. Note that TBS should be used for dilution, as PBS causes precipitation. Storage: When stored at 4°C, 0.1% sodium azide should be added as a preservative.Store the antibody at a minimum concentration of 2.0 mg/mL; a lower antibody concentration may result in decreased stability.The stock solution (2.0mg/mL) should be stored in aliquots at -20°C for 1year, or stored at 4°C for 6 months after adding 0.1% sodium azide. Avoid repeated freeze-thaw cycles. Diluted antibody should not be stored.