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  5. Invitrogen™ Mouse Eotaxin-1 (CCL11) ELISA Kit

Invitrogen™ Mouse Eotaxin-1 (CCL11) ELISA Kit

Artikelnummer. 18537874
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Menge:
10 x 96 Tests
5 x 96 Tests
96 Tests
Unit Size:
1 Set
1 Stück
10 Stück
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Product Code. 18537874

Brand: Invitrogen™ EMCCL11X10

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Description

ELISA

The Mouse Eotaxin-1/CCL11 ELISA quantitates Ms Eotaxin-1 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms Eotaxin-1. Principle of the method The Mouse Eotaxin-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

This gene is one of several Cys-Cys (CC) cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for eosinophils, but not mononuclear cells or neutrophils. This eosinophil specific chemokine assumed to be involved in eosinophilic inflammatory diseases such as atopic dermatitis, allergic rhinitis, asthma and parasitic infections.
TRUSTED_SUSTAINABILITY

Specifications

Zugriffsnummer P48298
Assay-Bereich 1.02 to 100 pg/mL
Assay-Empfindlichkeit: 1 pg/mL
Kalibrierbereich 1 pg/mL bis 100 pg/mL
Assay Festphasen-Sandwich-ELISA
Konjugat Biotin
Produkttyp ELISA-Kit
Proteinfamilie Chemokin und Rezeptoren
Probentyp Serum, Supernatant
Spezifität Dieses ELISA-Kit weist keine Kreuzreaktivität mit einem der getesteten Zytokine auf: Maus CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin-2, Fas Ligand, Fraktalkin, GCSF, GM-CFS, IFN-gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN (OB), LIX, L-Selektin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selektin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF)
Ausreichend für 96 Assays
Zielspezies Maus
Zur Verwendung mit (Anwendung) ELISA, Protein-Assays, Proteinbiologie, Krebsbiologie, Immunologie, Neurowissenschaften, Neurobiologie, Proteinnachweis, Proteinanalyse
Zur Verwendung mit (Geräte) Kolorimetrisches Mikrotiterplatten-Lesegerät
Gen-ID (Entrez) 20292
Gensymbol Scya11, Eotaxin
Interassay-VK <12%
Intraassay-VK <10
Kitinhalt Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Marker oder Farbstoff HRP
Menge 10 x 96 Tests
Kennzeichnung RUO
Forschungsgebiet Entzündungen
Probenvolumen Plasma 10 μL, Serum 10 μL, Supernatant 100 μL
Lagerungsbedingungen 2 bis 8 °C
Synonym Scya11, Eotaxin
Ziel Maus
Prüfzeit 1 h 20 min
Gesamte Assay-Zeit 4 h 45 min
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