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Gibco™ ExpiCHO™ Expression System Kit
Beschreibung
Beinhaltet
2 Fläschchen gefrorene ExpiCHO-S™ Zellen, 1 Flasche 1.000 ml ExpiCHO™ Expressionsmedium, 1 ExpiFectamine™ CHO-Transfektionskit für 1 l Kultur, 1 Fläschchen Positivkontrollvektor für die Antikörperexpression, 1 Flasche 100 ml OptiPRO™ SFM; alle Komponenten bei +2 °C bis +8 °C lagern, mit Ausnahme von gefrorenen Zellen, die in flüssigem Stickstoff gelagert werden
The ExpiCHO Expression System Kit combines the power of rapid, ultra–high-yield transient protein production in suspension culture with the benefit of expression in Chinese hamster ovary (CHO) cells. It is based on high-density culture of ExpiCHO-S cells in ExpiCHO Expression Medium. Transient expression is powered by ExpiFectamine CHO transfection reagent, designed specifically for high-density CHO suspension culture, in combination with a specialized transfection enhancer and culture feed.
All components work together synergistically with expression yields of 1–3 g per L of transfected culture demonstrated for some antibody and non-antibody proteins.
The ExpiCHO Expression System enables:
- Milligram to multigram yields of recombinant protein per liter of culture in 5–14 days post-transfection
- Robust and reproducible transfection results, for greater confidence in results and ability to use CHO cells from research to discovery
- Transfection efficiency of > 90% for high-density suspension CHO cell culture, with matching transfection enhancer and culture feed to boost transfection performance and protein expression
- Use of one-third to one-half less plasmid DNA than other transfection reagents
- Flexible, reliable culture and transfection protocols with multiple options that readily fit into current transient expression workflows
- Scalable expression for culture formats from <1 mL in deep well plates up to 5 L when using Nalgene angled-bottom shake flasks
The components included in the ExpiCHO Expression System Kit are: 2 vials of frozen ExpiCHO-S cells, 1 L of ExpiCHO Expression Medium, 1 ExpiFectamine CHO Transfection Kit sufficient to transfect 1 L of culture, OptiPRO SFM, and an antibody-expressing positive control vector.
ExpiCHO-S cells, ExpiCHO Expression Medium, ExpiFectamine CHO Transfection Kit, and OptiPRO SFM may also be purchased separately.
All components of the system are animal origin–free.
Tekniske data
Tekniske data
| Typ | Expressionssystem |
| Proteinmarkierung | Nicht markiert |
| Liefertyp | Transfektion |
| Menge | 1 kit |
| Inhalt und Lagerung | • 2 Röhrchen gefrorene ExpiCHO-S-Zellen • 1000 ml ExpiCHO Expressionsmedium • 1 ExpiFectamine CHO Transfektionskit für 1 l Kultur • 1 Fläschchen positiver Kontrollvektor zur Antikörper-Expression • 100 ml OptiPRO SFM Alle Komponenten bei 2 bis 8 °C lagern, mit Ausnahme von gefrorenen Zellen, die in flüssigem Stickstoff gelagert werden sollten. |
| Versandbedingung | Freigegeben für den Versand ohne Kühlung oder auf nassem Eis oder Trockeneis |
| Zelllinie | CHO-S, SFM angepasst |
| Expressionssystem | Säugetiere |
| Produktlinie | ExpiCHO™ |
| Zur Verwendung mit (Anwendung) | Proteinproduktion, konstitutive Expression |
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