Invitrogen™ F(ab)-Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody

F(ab)-Goat Recombinant Superclonal Secondary Antibody

F(ab)-Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody can detect all subtypes of Mouse IgG (Mouse IgG1,2a,2b). It showed minimum to no cross-reactivity to various species immunoglobulins tested. This Fab fragment antibody is expressed in vitro and purified as a recombinant construct. It is also engineered to deliver enhanced stability and sensitivity. The advantages of recombinant antibodies include-better specificity, animal origin-free formulation, and lot-to-lot consistency. F(ab) fragments offer several advantages over full-length IgGs due to their smaller molecular size (~50 kDa vs. ~150 kDa), such as improved tissue penetration. The absence of the Fc region reduces nonspecific binding and background staining in Fc receptor-expressing cells and tissues, while also enabling increased flexibility in multiplexing assays. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.