Invitrogen™ Gata-3 Monoclonal Antibody (TWAJ), eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Description: The monoclonal antibody TWAJ recognizes mouse and human Gata-3, a member of the Gata family of transcription factors. Gata-3 is a T cell-specific transcription factor important for thymic development and Th2 differentiation. Expression during embryonic development is found in the central nervous system, skin, mammary glands and kidney. During development, the expression of Gata-3 is essential as homozygous knock-out of Gata-3 is embryonic lethal. The Gata-3 is also essential for T cell commitment and survival. In the thymus, expression is found mainly on the CD4 single positive cells. During Th2 differentiation, Gata-3 binds to the IL-4 promoter as well as represses the expression of T-bet, thus inhibiting Th1 differentiation. Alternative splice variants have been reported especially in the MCF7 cell line. The TWAJ Human/Mouse Gata-3 antibody will recognize both forms (50 and 45 kDa) of the protein. Staining with the TWAJ Human/Mouse Gata-3 antibody requires the use of the Foxp3/Transcription Factor Staining Buffer Set.(cat 00-5523) Crossreactivity in rhesus monkeys has been published. Applications Reported: This TWAJ antibody has been reported for use in immunoblotting (WB) and immunocytochemistry. Applications Tested: This TWAJ antibody has been tested by western blotting of Th2 polarized human or mouse CD4 T cells or MCF7 cell line. This can be used at less than or equal to 2 μg/mL.