Invitrogen™ GFP Polyclonal Antibody, HRP

Rabbit Polyclonal Antibody

We offer the anti-GFP antibody HRP as purified rabbit IgG fraction and conjugated to horseradish peroxidase (HRP). At the time of preparation, the products are certified to be free of unconjugated dyes and are tested in a cytological experiment to ensure low nonspecific staining. This anti-GFP antibody HRP conjugate is suited for detection of native GFP, GFP variants, and most GFP-fusion proteins by western blot analysis and immunocytochemistry. For initial experiments, we recommend trying dilutions ranging from 1:200 to 1:2,000 for immunocytochemical applications and western blot analysis. It is a good practice to centrifuge the protein conjugate solutions briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step eliminates any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Storage and reconstitution: when stored desiccated at or below -20°C, the lyophilized powder is stable for at least one year. Reconstitute using 200 μL PBS, pH 7.2, to yield a 1 mg/mL stock solution containing 0.02% thimerosal. After reconstitution, store product at 4°C. For prolonged storage after reconstitution, add glycerol to a final concentration of 50% (v/v), aliquot, and store at or below -20°C. When stored properly, the solution is stable for approximately three months.