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PeproTech GM-CSF Polyclonal Antibody, PeproTech®, Invitrogen™

Beschreibung
AA Sequence of recombinant protein: MAPTRSPNPV TRPWKHVDAI KEALSLLNDM RALENEKNED VDIISNEFSI QRPTCVQTRL KLYKQGLRGN LTKLNGALTM IASHYQTNCP PTPETDCEIE VTTFEDFIKN LKGFLFDIPF DCWKPVQK. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Rat GM-CSF. Anti-Rat GM-CSF-specific antibody was purified by affinity chromatography employing an immobilized Rat GM-CSF matrix. Sandwich ELISA: To detect Rat GM-CSF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Rat GM-CSF (500-P225Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat GM-CSF. Western Blot: To detect Rat GM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Rat GM-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Spezifikation
Spezifikation
| Antigen | GM-CSF |
| Anwendungen | ELISA, Western Blot |
| Klassifikation | Polyclonal |
| Konzentration | 0.1-1.0 mg/mL |
| Konjugat | Unconjugated |
| Zusammensetzung | PBS with no preservative |
| Gen | CSF2 |
| Gen-Zugriffsnummer | P48750 |
| Gen-Alias | colony stimulating factor 2; colony stimulating factor 2 (granulocyte-macrophage); colony-stimulating factor; CSF; CSF2; Csfgm; cytokine; GMCSF; Gm-CSf; granulocyte macrophage colony stimulating factor; granulocyte macrophage-colony stimulating factor; granulocyte-macrophage colony stimulating factor 2; granulocyte-macrophage colony-stimulating factor; MGC131935; MGC138897; MGC151255; MGC151257; MGI-IGM; M-GM-CSF; molgramostin; put. GM-CSF; sargramostim |
| Gensymbole | CSF2 |
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