Invitrogen™ F(ab)-Goat anti-Human IgG (H+L) Superclonal™ Secondary Antibody

F(ab)-Goat Recombinant Superclonal Secondary Antibody

F(ab)-Goat anti-Human IgG (H+L), Superclonal™ Recombinant Secondary Antibody specifically detects human IgG Heavy and the Light chain with moderate cross reactivity to Rat IgG and Monkey IgG. No cross-reactivity was observed towards other species of immunoglobulins tested. Smaller F(ab) antibody fragments offer several benefits over whole IgGs, due to their smaller size (~50 kD vs. ~150 kD) and the absence of the Fc region. The smaller size is expected to allow conjugated F(ab) fragments to better penetration into tissue sections and improved staining in applications such as IHC. The lack of Fc region results in reduced nonspecific background staining when staining cells/ tissues with Fc receptors (such as immune cells) and other Fc interaction-related interference. Superclonal™ recombinant secondary antibodies are produced using a multifaceted clonal selection process that involves several phenotypic screens to ensure enhanced specificity, animal origin free formulation and lot-to-lot consistency. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.